Kadimastem - Stem Cell to Cure Diseases

Technology > High-Throughtput Technologies

High content screening (HCS), also known as visual screening analysis, is a method used in drug discovery to identify agents that alter the viewable morphological characteristics of cells under study.

In HCS, cells are first incubated with compounds of interest (small molecules, peptides or nucleic acids) and after a predefined period of time, structures, morphologies and molecular characteristics of the cells are analyzed.

The most common analysis involves staining of proteins with fluorescent tags, and using an automated imaging and analysis system to measure changes in cell expression of these  phenotype. The identification of small molecules that affect protein expressions and cellular functionality by converting them into a series of high-content analyzed images at different concentrations , is a key step in modern drug discovery. Using this technology has far-reaching implications for successfully identifying new drug candidates (a.k.a Hit compounds) and ultimately Lead compounds that may be advanced into clinical development.

 

Kadimastem’s drug screening system for research only currently includes the followings:

  • human neural stem cells (NSC) that commit into oligodendrocyte precursor cells (OPC commitment assay)
  • human OPC differentiation toward mature oligodendrocytes (differentiation assay)
  • human oligodendrocytes that myelinate neuron axons (myelination assay).
  • Beta cells/ Islets of Langerhans – human insulin producing cells assay